Part:BBa_G1004:Design

Forward BioBrick prefix primer for amplifying BioBrick parts (BioBrick-f)

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 9
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 9
  Illegal NotI site found at 15
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 9
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 9
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found in sequence at 9
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

It includes extra bases on the 5' end to enable both efficient cutting by EcoRI and 3' A addition by Taq polymerase.

Source

The BioBrick prefix.